Singh, DeepmalaDeepmalaSinghGhorpade, MohiniMohiniGhorpadeRegar, RamprasadRamprasadRegarCollot, MayeulMayeulCollotSoppina, VirupakshiVirupakshiSoppinaKanvah, SriramSriramKanvah2025-08-312025-08-312024-07-0110.1111/php.139102-s2.0-85186423952https://d8.irins.org/handle/IITG2025/2885938385897Fluorophores bearing cationic pendants, such as the pyridinium group, tend to preferentially accumulate in mitochondria, whereas those with pentafluorophenyl groups display a distinct affinity for the endoplasmic reticulum. In this study, we designed fluorophores incorporating pyridinium and pentafluorophenyl pendants and examined their impact on sub-cellular localization. Remarkably, the fluorophores exhibited a notable propensity for the mitochondrial membrane. Furthermore, these fluorophores revealed dual functionality by facilitating the detection of viscosity changes within the sub-cellular environment and serving as heavy-atom-free photosensitizers. With easy chemical tunability, wash-free imaging, and a favorable signal-to-noise ratio, these fluorophores are valuable tools for imaging mitochondria and investigating their cellular processes.falseCyanostilbenes | nystatin | pentafluorophenyl | sub-cellular imagingArticle17511097936-945July/August 20242arJournal1WOS:001169488700001