Jangra, SachinSachinJangraPurushothaman, GayathriGayathriPurushothamanJuvale, KapilKapilJuvaleRavi, SrimadhaviSrimadhaviRaviMenon, AishwaryaAishwaryaMenonThiruvenkatam, VijayVijayThiruvenkatamKirubakaran, SivapriyaSivapriyaKirubakaran2025-08-312025-08-312019-01-0110.2174/15680266196661902272123342-s2.0-85064008201https://d8.irins.org/handle/IITG2025/2343230827248Background & Objective: Helicobacter pylori infection is one of the primary causes of peptic ulcer followed by gastric cancer in the world population. Due to increased occurrences of multi-drug resistance to the currently available antibiotics, there is an urgent need for a new class of drugs against H. pylori. Inosine 5′-monophosphate dehydrogenase (IMPDH), a metabolic enzyme plays a significant role in cell proliferation and cell growth. It catalyses guanine nucleotide synthesis. IMPDH enzyme has been exploited as a target for antiviral, anticancer and immunosuppressive drugs. Recently, bacterial IMPDH has been studied as a potential target for treating bacterial infections. Differences in the structural and kinetic parameters of the eukaryotic and prokaryotic IMPDH make it possible to target bacterial enzyme selectively. Methods: In the current work, we have synthesised and studied the effect of substituted 3-aryldiazenyl indoles on Helicobacter pylori IMPDH (HpIMPDH) activity. The synthesised molecules were examined for their inhibitory potential against recombinant HpIMPDH. Results: In this study, compounds 1 and 2 were found to be the most potent inhibitors amongst the database with IC<inf>50</inf> of 0.8 ± 0.02µM and 1 ± 0.03 µM, respectively. Conclusion: When compared to the most potent known HpIMPDH inhibitor molecule C91, 1 was only four-fold less potent and can be a good lead for further development of selective and potent inhibitors of HpIMPDH.falseAzo compounds | Bacteria | Enzymatic studies | Helicobacter pylori | Indole | Inosine 5 ′-monophosphate dehydrogenase (IMPDH)Article18734294376-382201910arJournal10WOS:000465203100005