Kumar, SanjaySanjayKumarSharma, PramilaPramilaSharmaRatrey, PoonamPoonamRatreyDatta, BhaskarBhaskarDatta2025-08-302025-08-302016-07-0110.1007/s13197-016-2272-22-s2.0-84978700444https://d8.irins.org/handle/IITG2025/21869Nagpur mandarin orange (Citrus reticulata) peels were subjected to treatment with nanobiocatalysts in the form of cellulase and pectinase immobilized magnetic nanoparticles (MNPs). MNPs (Fe<inf>3</inf>O<inf>4</inf>) with average diameter in range of 40–90 nm were immobilized with cellulase and pectinase through APTES and glutaraldehyde. Treatment followed by extraction into organic solvents resulted in 8-9 fold increase in extraction of carotenoidic pigments compared to use of free enzymes. Optimum pH and temperature for the process were determined to be 5.0 and 50 °C, respectively. The nanobiocatalysts could be reused across three cycles with only 15 % drop in yield per cycle. Dinitrosalicylic acid assays showed that superior peel hydrolysis also led to greatest extent of pigment extraction.falseCarotenoids | Cellulase | Nanobiocatalysts | Pectinase | Pigment extractionArticlehttps://link.springer.com/content/pdf/10.1007/s13197-016-2272-2.pdf097584023013-30191 July 201618arJournal17WOS:000385186300012