Dubey, YogeshYogeshDubeyMansuri, ShabnamShabnamMansuriKanvah, SriramSriramKanvah2025-08-312025-08-312024-04-1610.1039/d4tb00353e2-s2.0-85191872650https://d8.irins.org/handle/IITG2025/2894938687117Iron, a crucial biologically active ion essential for metabolic processes in living organisms, plays a vital role in biological functions, and imbalances in iron levels can lead to various diseases. In this study, we have developed two simple “turn-on” fluorescent probes, NOPy and NOCN, for the quick and selective detection of Fe²⁺ at nanomolar levels (LOD of 35 nM), accompanied by significant absorption and emission shifts, along with colorimetric demarcation. Both fluorophores exhibit an excellent “turn-on” emission response upon encountering Fe²⁺ in the cells. Flow cytometry and confocal fluorescence imaging studies demonstrate enhanced fluorescence signals in response to labile iron, efficiently detecting heme during erastin-induced ferroptosis. Interestingly, we also observed that the product formed after Fe²⁺ sensing localizes within the lipid droplets. These water-soluble and highly sensitive reactive probes, NOPy and NOCN, enable investigations of iron-dependent physiological and pathological conditions. The development of these probes represents an advancement in the field, offering a rapid and selective means for detecting Fe²⁺ with minimal cytotoxicity.falseArticle205075184962-497416 April 20244arJournal5WOS:001210105300001