Patel, ManthanManthanPatelPatel, DivyeshDivyeshPatelDatta, SubhamoySubhamoyDattaSingh, UmashankarUmashankarSingh2025-08-312025-08-312020-07-2910.1186/s12863-020-00894-82-s2.0-85088851621https://d8.irins.org/handle/IITG2025/2408032727353Background: The human CGGBP1 binds to GC-rich regions and interspersed repeats, maintains homeostasis of stochastic cytosine methylation and determines DNA-binding of CTCF. Interdependence between regulation of cytosine methylation and CTCF occupancy by CGGBP1 remains unknown. Results: By analyzing methylated DNA-sequencing data obtained from CGGBP1-depleted cells, we report that some transcription factor-binding sites, including CTCF, resist stochastic changes in cytosine methylation. By analysing CTCF-binding sites we show that cytosine methylation changes at CTCF motifs caused by CGGBP1 depletion resist stochastic changes. These CTCF-binding sites are positioned at locations where the spread of cytosine methylation in cis depends on the levels of CGGBP1. Conclusion: Our findings suggest that CTCF occupancy and functions are determined by CGGBP1-regulated cytosine methylation patterns.trueAllelic imbalance | CGGBP1 | CTCF | Cytosine methylation | Stochasticity | Transcription factor binding sitesArticlehttps://bmcgenomdata.biomedcentral.com/track/pdf/10.1186/s12863-020-00894-81471215629 July 2020784arJournal13WOS:000557700500002