Agrawal, AdityaAdityaAgrawalVarshney, RajatRajatVarshneyPathak, MamtaMamtaPathakPatel, Shailesh KumarShailesh KumarPatelRai, VishalVishalRaiSulabh, SourabhSourabhSulabhGupta, RohiniRohiniGuptaSolanki, Khushal SinghKhushal SinghSolankiVarshney, RituRituVarshneyNimmanapalli, RamadeviRamadeviNimmanapalli2025-08-312025-08-312021-12-0110.1007/s13337-021-00737-92-s2.0-85114355459https://d8.irins.org/handle/IITG2025/25204Emerging pathogens have been an eternal threat to mankind. In a series of pandemics caused by notorious coronaviruses, a newly emerged SARS-CoV2 virus is creating panic among the world population. The unavailability of reliable theranostics insists the exploration of antigenic determinants in spike glycoprotein of SARS-CoV2. The four novel inserts (‘⁷⁰VSGTNGT⁷⁶’, ‘¹⁵⁰KSWM¹⁵³’, ²⁴⁷SYLTPG²⁵² and ⁶⁷⁴QTQTNSPRR⁶⁸²) in SARS-CoV2 spike protein were unraveled via multiple sequence alignment of spike proteins of SARS-CoV2, SARS-CoV, and MERS-CoV. The three-dimension (3D) modeling of the spike protein of the SARS-CoV2 and their interaction with the ACE2 receptor was delineated with the help of SWISS-MODEL and 3DLigandSite web servers. The predicted 3D model of SARS-CoV2 was further verified by SAVES, RAMPAGE, and ProSA-web tools. The potential B-cell immunogenic epitopes of SARS-CoV2 were predicted out by using various software viz. IEDB B-cell epitopes prediction tool, BepiPred linear epitope prediction tool, Emini Surface Accessibility Prediction tool, and Kolaskar-Tongaonkar antigenicity web tool. The five epitopes (i.e. ‘⁷¹SGTNGTKRFDN⁸¹, ²⁴⁷SYLTPG²⁵², ⁶³⁴RVYST⁶³⁸, ⁶⁷⁵QTQTNSPRRARSV⁶⁸⁷, and ¹⁰⁵⁴QSAPH¹⁰⁵⁸) were selected as potent antigenic determinants. The quantum of information generated by this study will prove beneficial for the development of effective therapeutics, diagnostics, and multi-epitopic vaccines to combat this ongoing menace.trueACE2 receptor | Epitopes | SARS-CoV2 | Spike protein | VaccinesArticlehttps://link.springer.com/content/pdf/10.1007/s13337-021-00737-9.pdf23473517774-783December 20212arJournal2