Singh, ParulParulSinghVithalani, HitashaHitashaVithalaniAdhyapak, AdityaAdityaAdhyapakSemwa, TinodaisheTinodaisheSemwaSingh, NihalNihalSinghDhanka, MukeshMukeshDhankaBhatia, DhirajDhirajBhatiaSaha, JhumaJhumaSaha2025-08-312025-08-312025-01-0110.1007/s10895-025-04140-12-s2.0-85217359122https://d8.irins.org/handle/IITG2025/28396Graphene quantum dots (GQDs) are highly valued for their chemical stability, tunable size, and biocompatibility. Utilizing green chemistry, a microwave-assisted synthesis method was employed to produce water-soluble GQDs from Mangifera Indica leaf extract. This approach is efficient, cost-effective, and environmentally friendly, offering reduced reaction times, energy consumption, and uniform particle sizes, and has proven advantageous over other methods. Water-soluble GQDs were synthesized using Mangifera Indica leaf extract, which ranged less than 15 nm in diameter, confirmed by high-resolution transmission electron microscopy with a lattice spacing of 0.34 nm. The GQDs exhibited strong photoluminescence with bright red fluorescence under UV light and excitation-independent emission at 662 nm with excitation wavelengths ranging from 300 to 500 nm, achieving a quantum yield of 10.3%. A peak at 27.2˚ was recorded corresponding to the graphite's (002) plane diffraction peak. Raman spectroscopy confirmed their graphitic nature and sp² crystallinity, with an intensity ratio of D and G peak I<inf>D</inf>/I<inf>G</inf> ratio of 1.12. Biocompatibility assays (MTT and live/dead) showed better results at lower concentrations (1 mg/ml) while higher concentrations (2 mg/ml) showed reduced efficacy. Antioxidant tests revealed increased DPPH scavenging activity with higher GQD concentrations and longer incubation times. The GQDs demonstrated excellent performance as fluorescent biosensors for Ni²⁺ (0.15 ppm) and Fe³⁺ (0.20 ppm), with high selectivity in river water samples, highlighting their potential for environmental and health applications.falseAntioxidant assay | Graphene quantum dots | Green synthesis | Live/dead assay | Metal-ion sensingArticle1573499420253arJournal1WOS:001399890000001