Wu, PeiwenPeiwenWuYu, YangYangYuMcGhee, Claire E.Claire E.McGheeTan, Li H.Li H.TanMishra, AbhijitAbhijitMishraWong, GerardGerardWongLu, YiYiLu2025-08-312025-08-312018-01-01[9783527339860, 9783527697106]10.1002/9783527697106.ch182-s2.0-105011000412https://d8.irins.org/handle/IITG2025/28530This chapter summarizes the applications of synchrotron-based spectroscopic techniques used to characterize nucleic acids. It reviews a few examples that illustrate some of the important principles governing the assembly of nucleic-acid-based materials systems. The chapter then focuses on the recent development and applications of small angle x-ray scattering (SAXS) in structural studies of nucleic acids. It also reviews the recent progress in using SAXS for characterizing DNA-based and DNA-functionalized 3D nanomaterial assemblies. The chapter also summarizes the progress made in using synchrotron-based spectroscopic techniques for the characterization of nucleic acids and related nucleic-acids-based or -templated nanomaterials. It describes time-resolved synchrotron X-ray footprinting in studying the folding of nucleic acid structures. The chapter covers the application of synchrotron radiation in studying DNA-based functional materials, including DNA nanostructures, such as DNA 3D origami structures and DNA-functionalized nanoparticle 3D assemblies, as well as DNA-lipid interactions.falseDNA-Functionalized Nanomaterials | nucleic acid structures | nucleic-acids-based nanomaterials | small angle x-ray scattering | synchrotron radiation | synchrotron-based spectroscopic techniques | time-resolved synchrotron x-ray footprintingBook Chapter687-7561 January 20180chBook0