Marapureddy, Sai GeethaSai GeethaMarapureddyHivare, PravinPravinHivareSharma, AarushiAarushiSharmaChakraborty, JuhiJuhiChakrabortyGhosh, SourabhSourabhGhoshGupta, SharadSharadGuptaThareja, PrachiPrachiThareja2025-08-312025-08-312021-10-0110.1016/j.carbpol.2021.1182542-s2.0-85108159812https://d8.irins.org/handle/IITG2025/2526434294291The direct write printing method has gained popularity in synthesizing scaffolds for tissue engineering. To achieve an excellent printability of scaffolds, a thorough evaluation of rheological properties is required. We report the synthesis, characterization, rheology, and direct-write printing of chitosan - graphene oxide (CH - GO) nanocomposite hydrogels at a varying concentration of GO in 3 and 4 wt% CH polymeric gels. Rheological characterization of CH - GO hydrogels shows that an addition of only 0.5 wt% of GO leads to a substantial increase in storage modulus (G′), viscosity, and yield stress of 3 and 4 wt% of CH hydrogels. A three-interval thixotropy test (3ITT) shows that 3 wt% CH with 0.5 wt% GO hydrogel has 94% recovery of G′ after 7 sequential stress cycles and is the best candidate for direct-write printing. Neuronal cell culture on 3 wt% CH with 0.5 wt% hydrogels reveals that GO promotes the differentiation of SH-SY5Y cells.falseChitosan | Direct write printing | Graphene oxide | Hydrogels | Rheology | SH-SY5Y cellsArticle1 October 202147118254arJournal49WOS:000678035900006