Singh, DeepmalaDeepmalaSinghShewale, Dipeshwari J.Dipeshwari J.ShewaleSengupta, AakashAakashSenguptaSoppina, VirupakshiVirupakshiSoppinaKanvah, SriramSriramKanvah2025-08-312025-08-312022-06-2410.1039/d2ob00995a2-s2.0-85134938779https://d8.irins.org/handle/IITG2025/2603535851393The mitochondria and endoplasmic reticulum (ER) are highly dynamic subcellular structures essential for several biological functions. The development of non-toxic, wash-free fluorophores to visualize these structures inside cells aid in understanding their localization and dynamics in diverse cellular processes. In this paper, we report the synthesis and characterization of lutidine-based cationic fluorophores bearing push-pull substituents exhibiting emission in green and red wavelength regions and their subcellular localization in living cells. The confocal imaging of the molecules in a cellular domain reveals the robust localization of three molecules (2, 4 and 5) in the mitochondria and two molecules with polyfluorophenyl substituents (6 and 7) in the ER. At the same time, the other two molecules (1 and 3) showed non-specific imaging. These molecules can also be used to sense the altered viscosity of the stressed ER and investigate its dynamics.falseArticle7047-705524 June 20228arJournal8WOS:000827852000001