Mukherjee, TarushyamTarushyamMukherjeeRegar, RamprasadRamprasadRegarSoppina, VirupakshiVirupakshiSoppinaKanvah, SriramSriramKanvah2025-08-312025-08-312021-12-1410.1039/d1ob01741a2-s2.0-85120748053https://d8.irins.org/handle/IITG2025/2517734610076The 'powerhouses' of cell, mitochondria have seen an upsurge of interest in investigations pertaining to the imaging and mapping of physiological processes. By utilizing sterol-modified rhodamine, we have performed the live-cell imaging of mitochondria without dependence on a membrane potential. The sterol probes are highly biocompatible, and they can track the mitochondrial live-cell dynamics in a background-free manner with improved brightness and impressive contrast. This is the first attempt to study the stress response using a direct fluorescence readout with bio-conjugates of rhodamine inside mitochondria. The results pave the way for developing different sterol markers for understanding cellular responses and function. This journal isfalseArticle10090-1009614 December 20215arJournal4WOS:000703639100001