Live-cell imaging of the nucleolus and mapping mitochondrial viscosity with a dual function fluorescent probe
Source
Organic and Biomolecular Chemistry
ISSN
14770520
Date Issued
2021-04-21
Author(s)
Mukherjee, Tarushyam
Soppina, Virupakshi
Ludovic, Richert
Mély, Yves
Klymchenko, Andrey S.
Collot, Mayeul
Abstract
Visualization of sub-cellular organelles allows the determination of various cellular processes and the underlying mechanisms. Herein, we report a fluorescent probe, bearing push-pull substituents emitting at 600 nm and its application in cellular imaging. The probe shows dual imaging of mitochondria and nucleoli and maps mitochondrial viscosity in live cells under various physiological variations and show minimum cytotoxicity. Nucleolar staining is confirmed by RNAase digestion.